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Rapid Detection of Salmonella in Bovine Lymph Nodes Using a Commercial Real-Time PCR Syst
     
 
Joshua Hill, Thomas S. Edrington, Guy H. Loneragan, Sara E. Gragg, David J. Nisbet
United States Department of Agriculture
USDA, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, College Station, Texas, 77845, USA
tom.edrington@ars.usda.gov
 Rapid Salmonella detection is needed to help prevent the distribution of contaminated food products. Using traditional culture methods, Salmonella detection can take up to 3-5 days. Using an improved protocol and a commercial real-time PCR system, we have shortened the detection time to less than 24 hr with comparable sensitivity and specificity to traditional Salmonella culture methods.
 
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