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Rapid Detection of Salmonella in Bovine Lymph Nodes Using a Commercial Real-Time PCR Syst
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Joshua Hill, Thomas S. Edrington, Guy H. Loneragan, Sara E. Gragg, David J. Nisbet
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United States Department of Agriculture
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USDA, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, College Station, Texas, 77845, USA
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tom.edrington@ars.usda.gov
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Rapid Salmonella detection is needed to help prevent the distribution of contaminated food products. Using traditional culture methods, Salmonella detection can take up to 3-5 days. Using an improved protocol and a commercial real-time PCR system, we have shortened the detection time to less than 24 hr with comparable sensitivity and specificity to traditional Salmonella culture methods.
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