Aspecific set of human genes is transcriptionally induced when cells areinfected with viruses or treated with interferons or dsRNA, the most prominentof which are the members of the ISG56 family. P56, the product of ISG56,interacts with human papilloma virus (HPV) E1 protein. E1 protein of HPV is oneof the key viral proteins that has enzymatic activities and is required forviral DNA replication. I have investigated the functional effect of P56interaction with the E1 protein. Transient DNA replication assays revealed thatHPV DNA replication is inhibited in a dose dependent manner by addition of IFN-b, which induces the expression of P56protein. Ablation of P56 expression by siRNA inhibited the effect of IFN.  Importantly, purified recombinant P56 proteincould inhibit HPV DNA replication invitro. I have identified the region of P56 that interacts with E1 proteinand inhibits viral DNA replication. Further studies with purified E1 proteinshow that P56 protein interferes with one of the key enzymatic activities of E1protein, the helicase activity. These findings demonstrate that interferonsensitivity of HPV replication is mediated by the IFN- inducible protein P56.

 1. Michifumi Yamachita, Saurabh Chattopadhya, Volker Fensterl, Paramananda Saikia, Jaime Wetzel and Ganes Sen ”EGF Receptor is Essential for TLR3 Signaling” Science Signaling, 5(233), ra50[DOI:10.11/26 scisignal 2002581] (2012)  [This paper is came as Editor Choice in Science Signaling]

2. Paramananda Saikia, Volker Fenstrel, Ganes C. Sen” The inhibitory action of P56 on select functions of E1 mediates interferon’s effect on HPV DNA replication” Journal of Virology,  Vol. 84, p-13036-13039 (2010)

3. Fulvia Terenzi*, Paramananda Saikia ^#, Ganes C. Sen “P56, an interferon-inducible protein, inhibits human papilloma virus DNA replication by interacting with the viral proteins E1” EMBO J, Vol. 27, p-3311-3321 (2008) # Co-First Author

4. Paramananda Saikia, M.S.Shaila. “Identification of functional domains of phosphoproteins of two morbilliviruses using chimeric proteins” Virus Genes, Vol. 37, p-1-8 (2008),

5.. Paramananda Saikia, Gopinath M, M.S. Shaila “Phosphorylation status of the phosphoprotein P of rinderpest virus modulates transcription and replication of the genome” Archives of Virology, Vol. 153, p-615-626 (2008)

Manuscript in review and in preparation:

1. Paramananda Saikia, Volker Fenstrel, Ganes C. Sen” Bovine P56 family of proteins inhibits Bovine papilloma virus DNA replication” Manuscript under preparation to be submitted to Journal of Virology (2012)

2. Michifumi Yamashita,  Carrie A. Millward, Hiroyuki Inoshita, Paramananda Saikia, Ganes Sen, Steven N. Emancipator “Viral Innate Immunity disturb podocyte cell function” manuscript in review in Journal of Innate Immunity.

CONFERENCE PUBLICATIONS:

· Ganes Sen, Michifumi Yamashita, Paramananda Saikia, Volker Fensterl, Christine White and Saurabh Chattopadhyay “Multiple antiviral pathways are activated by TLR3 and RIG-I Signaling” Vol. 52, p-70-71 Cytokine (2010) .

· Ganes Sen, Lu L, Fensterl Volker, White Christine, Michifumi Yamashita, Paramananda Saikia, Saurabh Chattopadhyay, Sarkar SN “Induction, functions and viral evasion of the ISG56 family of genes”  Cytokine 43(3) 234-234 (2008)