A Journal of Postdoctoral Research.
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    ISSN : 2328-9791
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Paf1C mediates regulation of gene expression by the MAP Kinase Slt2
Maria Soriano-Carot, Barbara Martinez-Bono, Inma Quilis and J. Carlos Igual
University of Valencia, and Boston University
Burjassot (Valencia), Spain AND Boston, MA, USA.

The budding yeast, Saccharomyces cerevisiae, has been widely used as a model organism to study the molecular mechanisms that regulate gene expression in eukaryotic cells. In the yeast Cell Wall Integrity Pathway (CWI), the protein Kinase C, Pkc1, activates the MAP Kinase Slt2, which in turn targets the transcription factors Rlm1 and SBF (Swi4-Swi6) and the transcriptional complex Paf1C, to modulate and control the expression of cell wall integrity genes. To better describe the connection between the CWI components and the transcriptional regulation of the cell integrity genes, a series of Chromatin Immunoprecipitation (ChIP) assays were performed. Our results reveal that the MAPK Slt2, associates to the promoter of several cell wall housekeeping genes like FKS1, MNN1 and GAS1. The expression of these genes is reduced in slt2 and pkc1 mutant strains. However, .....


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